short answer
Janoshik and Freedom Diagnostics rank first and second in Peptides Weekly's Q3 2026 lab guide. Janoshik has the broader menu (about 80 peptides, a blind GLP-1 test, many add-ons) and publishes an average of 7 business days. Freedom says it always pairs HPLC purity with LC-MS identity, and publishes an average of 1–3 business days. On one tesamorelin batch, a published comparison found 97.779% purity at Janoshik and 99.985% at Freedom: a single case, not proof that either number is wrong.
Janoshik vs Freedom Diagnostics in one table
Janoshik offers the broader menu and a blind GLP-1 test. Freedom Diagnostics sells HPLC purity and LC-MS identity together and publishes a shorter turnaround. The table compares what each lab lists on its own site and shows on its reports.
| Janoshik | Freedom Diagnostics | |
|---|---|---|
| Based in | Czech Republic | Franklin, Tennessee, US |
| Menu | About 80 peptides, plus steroids, SARMs and other compounds | Peptides and blends; does not accept HGH, HCG, HMG or testosterone products |
| Standard peptide test | HPLC: mg per vial and purity | HPLC purity with LC-MS identity; net content (mg) as an add-on |
| Identity shown on the report | The peptide is named; no identity evidence printed | Mass spectrum image; no expected mass or error value |
| Raw data on the report | Not on the standard report; sold as an add-on | Chromatogram and spectrum images; no peak table |
| Blind GLP-1 test | Yes: semaglutide, tirzepatide, retatrutide or a mixture | Not listed |
| Endotoxin | LAL, as an add-on or on its own | LAL per USP <85>, 2 replicates, reported as pass or fail |
| Sterility | Microbial count (TAMC and TYMC), pass or fail, 14 days | PCR screen for microbial DNA |
| Other add-ons | TFA, fentanyl check, variance across vials, pH, CHNS, LC-MS contamination screen | Extra vials on one report (conformity), fentanyl screen, pH on request |
| Vial photo | On the verify page, not on the report | On the report, at no charge |
| Published turnaround | Average 7 business days; sterility 14 days | Average 1–3 business days, from when the sample is logged in |
| Report check | Task number, 12-character key and QR code | Public lookup by company name or search code |
| Payment | Card, bank transfer, crypto | Card kept on file, charged when testing starts |
Neither lab claims ISO/IEC 17025 accreditation on its site. Neither prints a measurement uncertainty or a version number on the reports in our research.
What each lab does well
Janoshik. Breadth is the main strength: one lab for peptides, HGH (with dimer and larger proteins), steroids and SARMs. The blind GLP-1 test names which of semaglutide, tirzepatide or retatrutide is in the vial, or whether it is a mixture, without telling the lab the label. The key and QR open the lab’s own copy of each report, so a buyer can match any copy against it. Peptides Weekly ranks Janoshik first in its Q3 2026 lab guide, and Peptide Critic lists it as “Preferred”. More in our Janoshik review and Janoshik alternatives.
Freedom Diagnostics. Speed and identity are the main strengths. The lab says it never issues HPLC purity without LC-MS identity, and the report carries the lab’s own vial photo. Its public lookup lets anyone find a company’s reports. Freedom has more certificates than any other lab on Peptigrity (4,790) and Disclosed Labs (2,327). Peptides Weekly ranks it second. More in our Freedom Diagnostics review and Freedom Diagnostics alternatives.
How the rating sites list the two labs
Each site measures something different, so the rows do not add up to one verdict. Our guide to peptide rating sites explains each method.
| Rating site | Janoshik | Freedom Diagnostics |
|---|---|---|
| Finnrick: published tests (window) | 195 (Apr–Sep 2025) | 216 (Apr–Sep 2025) |
| Peptigrity: certificates / average purity | 2,556 / 99.5% | 4,790 / 99.5% |
| Disclosed Labs: COAs / average purity (%) ± SD | 1,035 / 99.56 ± 0.47 | 2,327 / 99.54 ± 1.27 |
| Merit Verified: lab score | 30, Limited | 70, Moderate |
| Peptides Weekly, Q3 2026 | Ranked first | Ranked second |
| Peptide Critic | Preferred | Standard |
Finnrick also shows a pass rate on each lab’s recent tests: 33% for Janoshik and 64% for Freedom. Those figures describe the vendor samples each lab received in 2025. The two labs tested different sets of vendor samples, so the pass rate is not a measure of lab accuracy. Merit Verified builds its lab score from factors that include accreditation, methods and independence.
Same batch, different numbers: the published cases
On 13 August 2026, the Substack writer Krysia published results for batches tested at more than one lab. These are single published cases, not a controlled study. Each lab received different vials from the same batch.
| Product (label) | Janoshik | Freedom Diagnostics | Other labs, same batch |
|---|---|---|---|
| Tesamorelin | 97.779% purity, 11.057 mg (average of three measurements) | 99.985% purity, 10.055 mg (average of two vials) | None reported |
| Epitalon (50 mg) | None reported | 99.935% purity, 47.3 mg | Chromate 95.915%, 55.5 mg; Accumark 99.27%, 83.64 mg (average of two vials) |
On tesamorelin, Freedom’s purity was 2.2 percentage points higher and its mg was 1 mg lower. On epitalon, the mg results ran from 47.3 to 83.64 mg on a 50 mg label. For more cases, see our report on same-batch comparisons.
Why two labs can disagree on one batch
Purity depends on what the software counts
HPLC purity is the main peak’s share of all UV peak area the lab detects. The wavelength, the column, the gradient and the integration settings all change which small peaks are counted. Printed digits are not the same as precision. In its answers to Finnrick’s method questionnaire, Krause Analytical says its values are valid to two significant figures.
The mg depends on the basis
Freeze-dried peptide powder also contains counter-ions (such as TFA or acetate) and water. Bachem’s worked example: 5 mg of powder with 85% net peptide content and 98.7% purity holds 4.195 mg of the target peptide. In Krysia’s 2 August 2026 piece, Janoshik said it standardises its own reference standards by CHNS and NMR, and reports net peptide without counter-ions. A lab that weighs total powder can report more mg. Peptigrity says that in September 2026 about 40% of single-compound certificates that report both figures were above 110% of label, and it marks the basis as not stated where a certificate does not give it. Our guide to net peptide content explains the gap.
Reference standards and single injections
Content per vial is measured against a reference standard. If two labs assign different content to their standards, every result shifts by the same proportion. Finnrick’s May 2026 blog describes one unnamed lab whose five reports were all off by the same proportion, which pointed to a calibration error. Some labs run one preparation and one injection per sample (Krause says so in its questionnaire answers), and vials in a batch differ.
How to compare the two labs on your own batch
- Take two vials from the same batch on the same day.
- Send one to each lab, and withhold the label amount if the lab allows it.
- Ask both labs which basis the mg uses (net peptide or total powder) and which reference standard they used.
- Ask for the chromatogram, and compare the peaks, not only the headline number.
What HK Analytical offers
We report purity (HPLC), identity (LC-MS) and content per vial, and we can add counter-ion (TFA, acetate) and water content (Karl Fischer), which explain much of the gap between powder weight and peptide. We also run endotoxin (LAL), blind GLP-1 identification, residual solvents and vial-to-vial consistency, in any mix per sample. Results within days of receipt. Sterility (USP <71>, 14 days) is the one test that takes 14 days, because the method incubates for 14 days.
Every number comes with its raw data, and each report is a signed record you can check on /verify. We do not hold ISO/IEC 17025 accreditation. Quotes are on request.
Questions
Is Janoshik or Freedom Diagnostics faster?
By their own published figures, Freedom Diagnostics: an average of 1–3 business days, counted from when the sample is logged in. Janoshik publishes an average of 7 business days for peptide tests and 14 days for its sterility add-on. Shipping time to each lab comes on top.
Why did Janoshik and Freedom give different purity for the same batch?
HPLC purity is the main peak's share of all peaks a lab counts, at its wavelength, on its column, with its integration settings. Two labs can count small peaks differently. The labs also tested different vials. One published case is not enough to say which number is right.
Does either lab run the USP <71> sterility test?
Neither lists it. Janoshik's sterility add-on is a microbial count (TAMC and TYMC), reported as pass or fail after 14 days. Freedom's is a PCR screen for microbial DNA. Both are screens with their own uses. USP <71> is a 14-day culture test of its own.
How do I verify a Janoshik or Freedom Diagnostics report?
For Janoshik, enter the task number and the 12-character key on its verification page, or scan the QR code. For Freedom, search its public lookup by company name or by search code (the first four characters of the company name plus the accession number). Compare every number and the vial photo with your copy.
Do Janoshik and Freedom report the mg the same way?
Not necessarily. Janoshik says it standardises its own reference standards and reports net peptide, without counter-ions. The Freedom reports in our research do not state a basis. A lab that weighs total powder can report more mg for the same vial. Ask each lab which basis its mg uses before you compare results.
Sources
- Janoshik: BPC-157 test page (method, turnaround, add-ons)
- Janoshik: GLP-1 blind test page
- Janoshik: sterility test page (TAMC and TYMC)
- Janoshik: report verification
- Janoshik: home page (test categories, payment methods)
- Freedom Diagnostics: home page and test list
- Freedom Diagnostics: sample intake form (tests, add-ons, products not accepted)
- Freedom Diagnostics: card authorisation page
- Freedom Diagnostics: public report lookup
- Freedom Diagnostics: example 2026 report
- Finnrick: Janoshik lab page
- Finnrick: Freedom Diagnostics lab page
- Krysia (Substack), 13 Aug 2026: tesamorelin and epitalon tested at several labs
- Krysia (Substack), 2 Aug 2026: same batch, three labs, with the labs' explanations
- Bachem: what net peptide content means
- Peptigrity: methodology
- Peptigrity: testing labs
- Disclosed Labs: labs
- Merit Verified: labs
- Peptides Weekly: an independent guide to the laboratories
- Peptide Critic: testing labs
- Krause Analytical: answers to Finnrick's method questionnaire
- Finnrick: independent peptide testing (calibration example)
Based on each lab’s public website and sample reports, checked September 2026. Prices, methods and turnaround change; check with the lab before you rely on them. Spotted an error? Write to contact@hkanalytical.com.