it answers
The share of UV-absorbing material, at the stated wavelength, that is the target peptide, and the main impurities by retention time.
it cannot tell you
- Water, salts, counter-ions, endotoxin, microbes or metals.
- The amount of peptide in the vial.
- High purity does not mean a vial is sterile or safe.
Method
High-performance liquid chromatography with UV detection. The main peak as a share of all peaks we detect, at the stated wavelength.
- technique
- HPLC-UV
- detector
- UV